Queensland's youth justice minister has accused the opposition of mudslinging and spurious allegations in state parliament, amid claims of a bad culture in her office.

Three chiefs of staff have left Gold Coast MP Laura Gerber's office since the LNP was elected almost 11 months ago.

Labor grilled the minister in question time, with Opposition Leader Steven Miles questioning if she has acted in line with her ministerial charter letter's directive to treat staff with "the highest levels of courtesy and respect".

"Will the minister guarantee to the house that they have never yelled at, hung up on, or acted inappropriately to public servants as reported in the media?" he asked.

Ms Gerber defended her conduct, accusing Labor in parliament of "trying to sling mud and make it stick".

"It's b

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