The Locust Valley school district is mandating its students use bathrooms and locker rooms that correspond to their biological sex, rather than gender identity — making the North Shore district at least the second on Long Island to adopt such policy.

The seven-member school board voted unanimously Wednesday to approve a resolution that changed an existing district policy , which said transgender or gender nonconforming students could use those spaces consistent with their “expressed gender identity.”

The new policy, which states students are not allowed to use a bathroom or locker room designated for the opposite sex, also applies to sports participation, the resolution said.

“The Resolution reflects this Board’s time-honored tradition of providing a safe, respectful and supportive

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