Rosie O’Donnell called out Jay Leno over a controversial moment in Netflix’s new “Biggest Loser” documentary.

The actress highlighted a moment from “Fit for TV: The Reality of the Biggest Loser” via Instagram Tuesday during which Leno spoke to contestant Tracey Yukich on his eponymous talk show in 2009.

During the “Tonight Show With Jay Leno” appearance, the now-75-year-old host read death threats Yukich had received live on air.

“jay leno is a mean a–hole #biggestloserdocumentary,” O’Donnell, 63, captioned the social media upload.

Leno’s rep has yet to respond to Page Six’s request for comment.

O’Donnell also blasted personal trainer Bob Harper on social media after watching the docuseries, which sheds light on the dark side of the weight-loss reality show.

“ok now bob harper

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