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Cancer Research UK has confirmed it is shutting down at least 88 stores by May 2026.
A further 100 are expected to follow by April 2027, making a total of nearly 200 locations set to close across the UK over the next 18 months.
Around 600 employees and as many as 3,000 volunteers could be affected by the closures.
Locations in Birmingham , Edinburgh and High Wycombe will form part of the initial wave of 88 shops shutting their doors.
Chief executive Michelle Mitchell stated: "Without action, we predict many of our shops will become unprofitable.
"A smaller, high-performing retail operation will mean we'll fund more life-saving cancer research and better serve our customers, staff and volunteers."
"Our decisions have been taken after serious consideration.
"We're commit